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Catalog No: OCOA06807
Size:20UG
Price: $179.00
SKU
OCOA06807
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SIRPA Protein Lysate (OCOA06807)

Datasheets/ManualsPrintable datasheet for SIRPA Protein Lysate (OCOA06807)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSIRPA HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSIRPA
Gene Full Namesignal regulatory protein alpha
Alias SymbolsBIT;Brain Ig-like molecule with tyrosine-based activation motifs;brain-immunoglobulin-like molecule with tyrosine-based activation motifs;CD172 antigen-like family member A;CD172A;inhibitory receptor SHPS-1;macrophage fusion receptor;MFR;MYD-1;myd-1 antigen;P84;PTPNS1;SHPS1;Signal-regulatory protein alpha-1;Signal-regulatory protein alpha-2;Signal-regulatory protein alpha-3;SIRP;tyrosine phosphatase SHP substrate 1;tyrosine-protein phosphatase non-receptor type substrate 1.
NCBI Gene Id140885
Protein NameNM_080792, NP_542970, P78324
Description of TargetHomo sapiens signal regulatory protein alpha (SIRPA), transcript variant 3, mRNA, Tyrosine-protein phosphatase non-receptor type substrate 1, tyrosine-protein phosphatase non-receptor type substrate 1 isoform 1 precursor [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P78324
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_542970
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_080792
Molecular Weight54.8 kDa
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