SIRPA Antibody (OASA01374)

Catalog No: OASA01374 (Formerly GWB-FEC272)

Size:200UG

Peripheral Human monocytes stained with Mouse anti Human CD172a:RPE

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: 15-414
• Isotype: IgG2a
• Host: Mouse
• Application: Flow cytometry|Immunohistochemistry-Frozen
• Additional Information: Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
  Histology Positive Control Tissue: Human tonsil.
• Reconstitution and Storage: -20°C
• Immunogen: Monocyte-derived dendritic cells.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: CD172a
• Application Info: FC: 1/25 - 1/00. Use 10 ul of the suggested working dilution to label 1x10⁶ cells in 100 ul.
  IHC-F: 1/100
• Application Data: Application #1: Peripheral Human monocytes stained with Mouse anti Human CD172a:RPE
• Reference: 1. Fujioka, Y. et al. (1996) A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion. Mol. Cell Biol. 16: 6887-6899.
  2. van Beek, E.M. et al. (2005) Signal regulatory proteins in the immune system. J. Immunol. 175: 7781-7787.
  

Target Info

• Gene Symbol: SIRPA
• Alias Symbols: BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1
• NCBI Gene Id: 140885
• Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• Description of Target: Mouse anti Human CD172a antibody, clone 15-414 recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.
  CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
• Uniprot ID: P78324
• Protein Accession #: NP_001035111.1
• Protein Size (# AA): 504