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Catalog No: OKEH01792
Size:96T
Price: $600.00
SKU
OKEH01792
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SIPA1L1 ELISA Kit (Human) (OKEH01792) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SIPA1L1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SIPA1L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SIPA1L1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
SIPA1L1 Microplate96 Wells (12 x 8 Well strips)
SIPA1L1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SIPA1L1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human Signal-induced proliferation-associated 1-like protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSIPA1L1
Gene Full Namesignal induced proliferation associated 1 like 1
Alias SymbolsE6TP1;high-risk human papilloma viruses E6 oncoproteins targeted protein 1;signal-induced proliferation-associated 1-like protein 1;SIPA1-like protein 1;SPAR1;spine-associated RapGAP 1.
NCBI Gene Id26037
Protein NameO43166
Description of TargetSignal-induced proliferation-associated 1-like protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/O43166
Protein Accession #NP_001271174.1
Nucleotide Accession #NM_001284245.1
Protein Size (# AA)1804
Molecular Weight200 kDa
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