Catalog No: OKEI00545
Size:96 Wells
Price: $625.00
SKU
OKEI00545
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Siglece ELISA Kit (Mouse) (OKEI00545) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Siglece has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Siglece is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Siglece | ||||||||||||||||||||||
ELISA Kit Range | 0.078 - 5 ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Siglece. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Siglece using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Biological Fluids | ||||||||||||||||||||||
Sensitivity | 46.9 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | SIGLECE |
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Alias Symbols | Sigl, Cd170, mSigle, Siglec5, Siglec9, Siglec12, Siglecl1, mSiglec-E |
NCBI Gene Id | 83382 |
Protein Name | Sialic acid-binding Ig-like lectin 12 |
Description of Target | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. |
Uniprot ID | Q91Y57 |
Protein Accession # | NP_112458 |
Nucleotide Accession # | NM_031181 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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