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Catalog No: OKEI00545
Size:96 Wells
Price: $625.00
SKU
OKEI00545
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Siglece ELISA Kit (Mouse) (OKEI00545) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Siglece has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Siglece is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Siglece
ELISA Kit Range0.078 - 5 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Siglece. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Siglece using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
LACTB2 Microplate96 Wells (12 x 8 Well strips)
LACTB2 Lyophilized Standard2
100X LACTB2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity46.9 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSIGLECE
Alias SymbolsSigl, Cd170, mSigle, Siglec5, Siglec9, Siglec12, Siglecl1, mSiglec-E
NCBI Gene Id83382
Protein NameSialic acid-binding Ig-like lectin 12
Description of TargetPutative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.
Uniprot IDQ91Y57
Protein Accession #NP_112458
Nucleotide Accession #NM_031181
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