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Datasheets/Manuals | Printable datasheet for SIAH1 Antibody (OAEB01633) |
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Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
:: | Description: This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1) |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | SIAH1 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 86%; Rat: 100%; Zebrafish: 100% |
Peptide Sequence | SRQTATALPTGTSKC |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 28+30kDa bands observed in Human Liver lysates (calculated MW of 31.1kDa and 34.6kDa according to NP_003022.3 and NP_001006611.1). Recommended concentration: 0.1-0.3ug/ml. |
Reference | * Guigon CJ, Zhao L, Lu C, Willingham MC, Cheng SY.; Regulation of beta-catenin by a novel nongenomic action of thyroid hormone beta receptor.; Mol Cell Biol. 2008 Jul;28(14):4598-608.; Pubmed 18474620 * Groenman F, Rutter M, Caniggia I, Tibboel D, Post M.; Hypoxia-inducible factors in the first trimester human lung.; J Histochem Cytochem. 2007 Apr;55(4):355-63.; Pubmed 17189520 * Iwai A, Marusawa H, Matsuzawa S, Fukushima T, Hijikata M, Reed JC, Shimotohno; K, Chiba T.; Siah-1L, a novel transcript variant belonging to the human Siah family of proteins, regulates beta-catenin activity in a p53-dependent manner.; Oncogene. 2004 Sep 30;23(45):7593-600.; Pubmed 15326481 * Susini L, Passer BJ, Amzallag-Elbaz N, Juven-Gershon T, Prieur S, Privat N, Tuynder M, Gendron MC, Israel A, Amson R, Oren M, Telerman A.; Siah-1 binds and regulates the function of Numb.; Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15067-72.; Pubmed 11752454 |
Gene Symbol | Siah1|SIAH1|Siah1a |
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Gene Full Name | siah E3 ubiquitin protein ligase 1|siah E3 ubiquitin protein ligase 1A |
Alias Symbols | AA982064;AI853500;BURHAS;E3 ubiquitin-protein ligase SIAH1;E3 ubiquitin-protein ligase SIAH1A;mSiah-1a;RING-type E3 ubiquitin transferase SIAH1;RING-type E3 ubiquitin transferase SIAH1A;seven in absentia 1A;seven in absentia homolog 1;seven in absentia homolog 1a;Si;SIAH;siah-1;siah-1a;SIAH1A;Sinh1a. |
NCBI Gene Id | 140941|20437|6477 |
Protein Name | E3 ubiquitin-protein ligase SIAH1 |
Uniprot ID | Q8IUQ4 |
Protein Accession # | NP_003022.3 |
Molecular Weight | 31.1; 34.6 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SIAH1 Antibody (OAEB01633)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Rat".
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How long will it take to receive "SIAH1 Antibody (OAEB01633)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "SIAH1 Antibody (OAEB01633)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SIAH1 Antibody (OAEB01633)"?
This target may also be called "AA982064;AI853500;BURHAS;E3 ubiquitin-protein ligase SIAH1;E3 ubiquitin-protein ligase SIAH1A;mSiah-1a;RING-type E3 ubiquitin transferase SIAH1;RING-type E3 ubiquitin transferase SIAH1A;seven in absentia 1A;seven in absentia homolog 1;seven in absentia homolog 1a;Si;SIAH;siah-1;siah-1a;SIAH1A;Sinh1a." in publications.
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What is the shipping cost for "SIAH1 Antibody (OAEB01633)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SIAH1 Antibody (OAEB01633)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SIAH1 Antibody (OAEB01633)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "31.1; 34.6".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SIAH1 Antibody (OAEB01633)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Siah1|SIAH1|Siah1a"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Siah1|SIAH1|Siah1a"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Siah1|SIAH1|Siah1a"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Siah1|SIAH1|Siah1a"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Siah1|SIAH1|Siah1a"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Siah1|SIAH1|Siah1a"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.