SHOC2 Antibody (OAPB00903)

Catalog No: OAPB00903

Size:100UG

Western blot analysis of SHOC2 in Jurkat cell lysate with SHOC2 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of SHOC2 in human spleen tissue with SHOC2 antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Human|Mouse|Rat
• Product Format: Liquid. SHOC2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: SHOC2 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: SHOC2 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human SHOC2. The immunogen is located within amino acids 30 - 80 of SHOC2.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-SHOC2 antibody is Catalog # OEPB03025
• Application Info: SHOC2 antibody can be used for detection of Shoc2 by Western blot at 1-2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Selfors LM, Schutzman JL, Borland CZ, et al. Soc-2 encodes a leucine-rich repeat protein implicated in fibroblast growth factor receptor signaling. Proc. Natl. Acad. Sci. USA1998; 95:6903-8.;Sieburth DS, Sun Q, and Han M. SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans. Cell1998; 94:119–130.;Rodriguez-Viciana P, Oses-Prieto J, Burlingame A, et al. A phosphatase holoenzyme comprised of Shoc2/Sur8 and the catalytic subunit of PP1 functions as an M-Ras effector to modulate Raf activity. Mol. Cell2006; 22:217-30.;Li W, Han M, and Guan KL. The leucine-rich repeat protein SUR-8 enhances MAP kinase activation and forms a complex with Ras and Raf. Genes Dev.2000; 14:895–900.

Target Info

• Gene Symbol: SHOC2
• Gene Full Name: SHOC2 leucine rich repeat scaffold protein
• Alias Symbols: leucine-rich repeat protein SHOC-2;NSLH1;Protein soc-2 homolog;Protein sur-8 homolog;SIAA0862;SOC2;soc-2 suppressor of clear homolog;SUR8.
• NCBI Gene Id: 8036
• Protein Name: Leucine-rich repeat protein SHOC-2|leucine-rich repeat protein SHOC-2 isoform 1 [Homo sapiens]
• Description of Target: Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.
• Uniprot ID: Q9UQ13
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_031399