SHMT2 Antibody (OAGA07185)

Catalog No: OAGA07185

Size:100 ul

Sample (whole cell lysate)
A: 293T 10ug
B: HeLa 10ug
7.5% SDS PAGE
diluted at 1:10000

SHMT2 antibody detects SHMT2 protein by Western blot analysis.
A. 30 ug A431 whole cell lysate/extract
B. 30 ug HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
SHMT2 antibody dilution: 1:5000

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using SHMT2 antibody at 1:500 dilution.

SHMT2 antibody detects SHMT2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 1 sec.
Green: SHMT2 protein stained by SHMT2 antibody diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SHMT2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: SHMT2
• Gene Full Name: serine hydroxymethyltransferase 2
• Alias Symbols: GLYA, SHMT, NEDCASB, HEL-S-51e
• NCBI Gene Id: 6472
• Protein Name: serine hydroxymethyltransferase, mitochondrial
• Description of Target: This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
• Uniprot ID: P34897
• Protein Accession #: NP_001159828.1
• Nucleotide Accession #: NM_001166356.1