SHH Protein (OPPA02401)

Catalog No: OPPA02401 (Formerly GWB-BSP067)

Size:5UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: SHH Mouse His Tag solution containing 20mM Trsi HCL pH-8, 1mM DTT, and 10% glycerol. Physical appearance: Sterile filtered colorless solution.
• Host: E. Coli
  Product Introduction: Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.
  Product Description: SHH Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 183 amino acids (25-198 a.a.) and having a molecular mass of 20.8 kDa. SHH protein is fused to a 8 amino acid His-Tag at C-terminus and purified by standard chromatography.
• Reconstitution and Storage: SHH Mouse although stable at 4C for 1 week, should be stored desiccated below -18C. Please prevent freeze thaw cycles.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Peptide Sequence: MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH.

Target Info

• Gene Symbol: SHH
• Alias Symbols: Hx, Dsh, Hhg1, Hxl3, ShhNC, M100081, 9530036O11Rik
• NCBI Gene Id: 20423
• Protein Name: Sonic hedgehog protein
• Description of Target: Recombinant Mouse Sonic HedgeHog, His Tag
• Uniprot ID: Q62226
• Protein Accession #: NP_033196.1
• Protein Size (# AA): Recombinant