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Catalog No: OPPA01657 (Formerly GWB-EB4591)
Size:5UG
Price: $75.00
SKU
OPPA01657
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SHH Protein (OPPA01657)

Datasheets/ManualsPrintable datasheet for OPPA01657
Product Info
Predicted Species ReactivityMouse
Product FormatLyophilized from a concentrated (1mg/ml) solution in water containing 10mM sodium Phosphate buffer pH-7.5. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
HostE. Coli
Additional InformationSolubility: It is recommended to reconstitute the lyophilized Sonic HedgeHog in sterile 18M Omega -cm H2O at a concentration of 0.1mg-0.5mg per 1ml.
::Biological Activity: Determined by it's ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED50 in the range of 0.05 - 0.25 ug/ml.
::Product Introduction: Recombinant Mouse Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.
Product Description: Sonic Hedgehog Recombinant Mouse produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8 kDa. The Mouse Sonic Hedgehog is 99% homologous to the human gene.Cysteine at position 25 has been substituted with Ile.The Sonic HedgeHog is purified by proprietary chromatographic techniques.
Reconstitution and StorageLyophilized Mouse Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Sonic HedgeHog should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles.
PurityGreater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Peptide SequenceMIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG.
Gene SymbolmSHH
Alias SymbolsHx, Dsh, Hhg1, Hxl3, ShhNC, M100081, 9530036O11Rik
NCBI Gene Id20423
Protein NameSonic hedgehog protein
Description of TargetRecombinant Mouse Sonic HedgeHog
Uniprot IDQ62226
Protein Accession #NP_033196.1
Protein Size (# AA)Recombinant
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