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Catalog No: OKCD07711
Size:96WELLS
Price: $825.00
SKU
OKCD07711
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Datasheets/ManualsPrintable datasheet for SHH ELISA Kit (Rat) (OKCD07711)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Sonic (SHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Homolog, Sonic (SHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Homolog, Sonic (SHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hedgehog Homolog, Sonic (SHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SHH Microplate96 Wells (12 x 8 Well strips)
SHH Lyophilized Standard2
100X SHH HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.59ng/mL
Gene SymbolShh
Gene Full Namesonic hedgehog signaling molecule
Alias Symbolsshh unprocessed N-terminal signaling and C-terminal autoprocessing domains;ShhNC;sonic hedgehog homolog;sonic hedgehog protein.
NCBI Gene Id29499
Protein NameSonic hedgehog protein
Description of TargetSonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity).
Uniprot IDQ63673
Protein Accession #NP_058917.1
Nucleotide Accession #NM_017221.1
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