Catalog No: OKCA02454
Size:96 Wells
Price: $754.00
SKU
OKCA02454
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8380-85
1:29188-93
1:4102100-104
1:88682-90
ELISA Kit PrincipleAviva Systems Biology Shh ELISA Kit (Mouse) (OKCA02454) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Shh has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Shh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Shh captured in the well.
ELISA Kit Range28 - 1,800 pg/mL
ELISA Kit Recovery
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)104100-107
EDTA Plasma (n=4)9087-93
ELISA Kit ReproducibilityMean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
ELISA Kit Component
ComponentAmount
Shh Microplate96 Wells (12 x 8 Well strips)
Shh Lyophilized Standard2
100X Biotinylated Shh Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity7 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSHH
Alias SymbolsHx, Dsh, Hhg1, Hxl3, ShhNC, M100081, 9530036O11Rik
NCBI Gene Id20423
Protein NameSonic hedgehog protein
Description of TargetIntercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO.
Uniprot IDQ62226
Protein Accession #NP_033196
Nucleotide Accession #NM_009170
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