SHANK3 Antibody (OASE00184)

Catalog No: OASE00184 (Formerly GWB-9124BA)

Size:100ug

Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.

Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S69-46
• Isotype: IgG2b
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, AM
• Additional Information: Background Info: Detects ~190kDa. No cross-reactivity against Shank1 or Shank2.
  Scientific Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (3). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling; specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (4).
  Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (5).
  Certificate of Analysis: 1ug/mL was sufficient for detection of Shank3 in 10ug COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Synthetic peptide amino acids 840-857 of rat Shank3
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~190kDa. No cross-reactivity against Shank1 or Shank2.
• Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
  2. www.iochannels.org
  3. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856.
  4. Schuetz G., et al. (2004) JCB. 167(5): 645-952.
  5. Durand C.M., et al. (2007) Nature Genetics. 39: 25-27.
• Description: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling; specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (2). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (3).

Target Info

• Gene Symbol: SHANK3
• Gene Full Name: SH3 and multiple ankyrin repeat domains 3
• Alias Symbols: AI841104, DEL22q13.3, KIAA1650, Proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, SH3 and multiple ankyrin repeat domains 3, SH3 and multiple ankyrin repeat domains protein 3 SH3/ankyrin domain gene 3, SHAN3_HUMAN, Shank postsynaptic density protein, Shank3, Shank3b, SPANK 2, SPANK2
• NCBI Gene Id: 59312
• Uniprot ID: Q9JLU4
• Protein Accession #: NP_067708.1