- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OASE00306-HRP |
---|
Predicted Species Reactivity | Mouse, Rat |
---|---|
Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | S367-51 |
Isotype | IgG2a |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB, ICC, IHC |
Additional Information | Background Info: Detects a 190 kDa protein. Cross-reacts with SHANK1. Does not cross-react with SHANK2. |
:: | Scientific Background: SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. |
:: | Certificate of Analysis: 1ug/mL was sufficient for detection of SHANK1/SHANK3 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3 (accession no. Q9JLU4) Mouse: 100% identity (89/89 amino acids identical) Human: 97% identity (87/89 amino acids identical) ~70% identity with SHANK1 and SHANK2 |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 1:1000 WB |
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
Gene Symbol | Shank3 |
---|---|
Gene Full Name | SH3 and multiple ankyrin repeat domains 3 |
Alias Symbols | Prosap2, Spank-2 |
NCBI Gene Id | 59312 |
Uniprot ID | Q9JLU4 |
Protein Accession # | NP_067708.1. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rat".
-
How long will it take to receive "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
-
What buffer format is "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)"?
This target may also be called "Prosap2, Spank-2" in publications.
-
What is the shipping cost for "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SHANK1/SHANK3 Antibody : HRP (OASE00306-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "SHANK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "SHANK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "SHANK3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "SHANK3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "SHANK3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "SHANK3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.