SHANK (pan) Antibody (OASE00175)

Catalog No: OASE00175 (Formerly GWB-38E8A0)

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S23b-49
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, IP, ICC, IF, AM
• Additional Information: Background Info: Detects ~160kDa. Recognizes Shank1, 2 and 3.
  Scientific Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of
  membrane and cytoplasmic proteins (3).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Shank1-4 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 84-309 of rat Shank2
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~160kDa. Recognizes Shank1, 2 and 3.
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
  2. www.iochannels.org
  3. Sheng M., and Kim E. (2000) Journal of Cell Science.
  113: 1851-1856.
• Description: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (3).

Target Info

• Gene Symbol: SHANK
• Gene Full Name: SH3 and multiple ankyrin repeat domains 2
• Alias Symbols: Cortactin binding protein 1, Cortactin SH3 domain-binding protein, Cortactin-binding protein 1, CortBP1, CTTNBP1, GKAP/SAPAP interacting protein, GKAP/SAPAP-interacting protein, KIAA1022, Proline rich synapse associated protein 1, Proline-rich synapse-associated protein 1, ProSAP1, SH3 and multiple ankyrin repeat domains protein 2, SHAN2_RAT, SHANK, Shank2, SPANK-3
• NCBI Gene Id: 171093
• Uniprot ID: Q9QX74