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Datasheets/Manuals | Printable datasheet for anti-SH3RF1 (ARP43283_P050-HRP) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human SH3RF1 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
Peptide Sequence | Synthetic peptide located within the following region: LLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHG |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-SH3RF1 (ARP43283_P050-HRP) antibody is Catalog # AAP43283 (Previous Catalog # AAPP11358) |
Reference | Lyons,T.R., (2007) J. Biol. Chem. 282 (30), 21987-21997 |
Gene Symbol | SH3RF1 |
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Gene Full Name | SH3 domain containing ring finger 1 |
Alias Symbols | POSH, RNF142, SH3MD2 |
NCBI Gene Id | 57630 |
Protein Name | E3 ubiquitin-protein ligase SH3RF1 |
Description of Target | SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. |
Uniprot ID | Q7Z6J0 |
Protein Accession # | NP_065921 |
Nucleotide Accession # | NM_020870 |
Protein Size (# AA) | 888 |
Molecular Weight | 93kDa |
Protein Interactions | YWHAB; UBE2N; UBE2D3; UBE2D1; AKT2; AKT1; SIAH2; SIAH1; SH3RF2; RAC1; SEMA6A; UBC; PDCD6IP; KCNJ1; MAP4K3; MAP3K9; ADAM15; PAK2; RACGAP1; MAP3K12; MAP3K10; MAP3K11; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit".
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How long will it take to receive "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)"?
This target may also be called "POSH, RNF142, SH3MD2" in publications.
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What is the shipping cost for "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "93kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SH3RF1 Antibody - middle region : HRP (ARP43283_P050-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SH3RF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SH3RF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SH3RF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SH3RF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SH3RF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SH3RF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.