SH3BGRL3 ELISA Kit (Human) (OKEH08640)

Catalog No: OKEH08640

Size:96T

Human SH3BGRL3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SH3BGRL3 ELISA Kit (Human) (OKEH08640) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SH3BGRL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SH3BGRL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SH3BGRL3 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.7% (n = 20)
  Mean Inter-assay CV%: <=9.1% (n = 20)
• ELISA Kit Component:
  • SH3BGRL3 Microplate: 96 Wells (12 x 8 Well strips)
  • SH3BGRL3 Lyophilized Standard: 2
  • 100X Biotinylated SH3BGRL3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Could act as a modulator of glutaredoxin biological activity.
  Subcellular Location: Cytoplasm Nucleus
  Unigene: Hs.109051
  SMR: Q9H299
  String: 9606.ENSP00000270792
  KEGG: hsa:83442
  MIM: 615679
  Pfam: PF04908
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 20pg/mL
• Specificity: Natural and recombinant Human SH3 domain-binding glutamic acid-rich-like protein 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SH3BGRL3
• Gene Full Name: SH3 domain binding glutamate rich protein like 3
• Alias Symbols: epididymis secretory protein Li 297;epididymis secretory sperm binding protein;HEL-S-297;SH3 domain binding glutamic acid-rich protein like 3;SH3 domain-binding glutamic acid-rich-like protein 3;SH3 domain-binding protein 1;SH3BGRL3-like protein;SH3BP-1;TIP-B1;TNF inhibitory protein.
• NCBI Gene Id: 83442
• Protein Name: Q9H299
• Description of Target: SH3 domain-binding glutamic acid-rich-like protein 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q9H299
• Protein Accession #: NP_112576.1
• Nucleotide Accession #: NM_031286.3