Catalog No: OKCD00631 (Formerly GWB-KBBPT6)
Size:96WELLS
Price: $750.00
SKU
OKCD00631
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for SH3BGRL ELISA Kit (Human) (OKCD00631) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.061 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL). No significant cross-reactivity or interference between SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | SH3BGRL |
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Gene Full Name | SH3 domain binding glutamate rich protein like |
Alias Symbols | epididymis secretory protein Li 115;HEL-S-115;SH3 domain binding glutamic acid-rich protein like;SH3 domain-binding glutamic acid-rich-like protein;SH3BGR;SH3-binding domain glutamic acid-rich protein like. |
NCBI Gene Id | 6451 |
Protein Name | SH3 domain-binding glutamic acid-rich-like protein |
Description of Target | SH3 domain-binding glutamic acid-rich-like protein |
Uniprot ID | O75368 |
Protein Accession # | NP_003013 |
Nucleotide Accession # | NM_003022.2 |
Protein Size (# AA) | 114 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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