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Catalog No: OKCD02853
Size:96WELLS
Price: $725.00
SKU
OKCD02853
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Datasheets/ManualsPrintable datasheet for SGPP1 ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SGPP1 Microplate96 Wells (12 x 8 Well strips)
SGPP1 Lyophilized Standard2
100X Biotinylated SGPP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). No significant cross-reactivity or interference between Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSGPP1
Gene Full Namesphingosine-1-phosphate phosphatase 1
Alias SymbolsSphingosine-1-phosphatase 1, Sphingosine-1-phosphate phosphatase 1, Spp1, SPPase1
Protein NameSphingosine-1-phosphate phosphatase 1
Description of TargetHas enzymatic activity against both sphingosine 1-phosphate (S1P) and dihydro-S1P. Regulates intracellular and extracellular S1P levels.
Uniprot IDQ99P55
Protein Accession #XP_343082.4
Nucleotide Accession #XM_343081.8
Protein Size (# AA)430
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