Catalog No: OKCD02853
Size:96WELLS
Price: $725.00
SKU
OKCD02853
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for SGPP1 ELISA kit (Rat) |
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Predicted Species Reactivity | Rat | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colormetric|Regular detection | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C, -20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.057 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). No significant cross-reactivity or interference between Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | SGPP1 |
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Gene Full Name | sphingosine-1-phosphate phosphatase 1 |
Alias Symbols | Sphingosine-1-phosphatase 1, Sphingosine-1-phosphate phosphatase 1, Spp1, SPPase1 |
Protein Name | Sphingosine-1-phosphate phosphatase 1 |
Description of Target | Has enzymatic activity against both sphingosine 1-phosphate (S1P) and dihydro-S1P. Regulates intracellular and extracellular S1P levels. |
Uniprot ID | Q99P55 |
Protein Accession # | XP_343082.4 |
Nucleotide Accession # | XM_343081.8 |
Protein Size (# AA) | 430 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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