SGK1 Antibody (Phospho-Ser78) (OASG06603)

Catalog No: OASG06603

Size:100 uL

Western Blot using 293

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, ELISA
• Additional Information: Tissue Specificity: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human SGK1 around the phosphorylation site of S78. Location: 10-90aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Regulated by phosphorylation. Activated by phosphorylation on Ser-422 by mTORC2, transforming it into a substrate for PDPK1 which phosphorylates it on Thr-256. Phosphorylation on Ser-397 and Ser-401 are also essential for its activity. Phosphorylation on Ser-78 by MAPK7 is required for growth factor-induced cell cycle progression. Ubiquitinated by NEDD4L.which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum.which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability.
• Specificity: Phospho-SGK1 (S78) Polyclonal Antibody detects endogenous levels of SGK1 protein only when phosphorylated at S78.
• Application Info: WB: 1:500~2000
  ELISA: 1:10000

Target Info

• Gene Symbol: SGK1
• Gene Full Name: serum/glucocorticoid regulated kinase 1
• Alias Symbols: SGK
• NCBI Gene Id: 6446; 20393;
• Description of Target: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
• Uniprot ID: O00141, Q9WVC6, Q06226