Catalog No: OKCD01259 (Formerly GWB-KBBUI8)
Size:96WELLS
Price: $675.00
SKU
OKCD01259
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Datasheets/ManualsPrintable datasheet for SGCD ELISA Kit (Human) (OKCD01259)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sarcoglycan Delta (SGCd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sarcoglycan Delta (SGCd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sarcoglycan Delta (SGCd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sarcoglycan Delta (SGCd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.781-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SGCD Microplate96 Wells (12 x 8 Well Strips)
SGCD Lyophilized Standard2 x 50 ng
100X Biotinylated SGCD Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.28 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sarcoglycan Delta (SGCd). No significant cross-reactivity or interference between Sarcoglycan Delta (SGCd) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSGCD
Gene Full Namesarcoglycan delta
Alias Symbols35 kDa dystrophin-associated glycoprotein;35DAG;CMD1L;DAGD;delta-sarcoglycan;delta-SG;dystrophin associated glycoprotein, delta sarcoglycan;LGMDR6;placental delta sarcoglycan;sarcoglycan, delta (35kDa dystrophin-associated glycoprotein);SGCDP;SGD;SG-delta.
NCBI Gene Id6444
Protein NameDelta-sarcoglycan
Description of TargetComponent of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Uniprot IDQ92629
Protein Accession #NP_000328
Nucleotide Accession #NM_000337.5
Protein Size (# AA)289
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