SFTPD ELISA Kit (Human) (OKCD07083)

Catalog No: OKCD07083

Size:96WELLS

SFTPD ELISA Kit (Human) (OKCD07083) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein D (SPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Associated Protein D (SPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein D (SPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Surfactant Associated Protein D (SPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Associated Protein D (SPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Associated Protein D (SPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SFTPD Microplate: 96 Wells (12 x 8 Well strips)
  • SFTPD Lyophilized Standard: 2
  • 100X SFTPD HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
• Sensitivity: < 0.233ng/mL

Target Info

• Gene Symbol: SFTPD
• Gene Full Name: surfactant protein D
• Alias Symbols: COLEC7;collectin-7;lung surfactant protein D;PSP-D;pulmonary surfactant apoprotein;pulmonary surfactant-associated protein D;SFTP4;SP-D;surfactant-associated protein, pulmonary 4.
• NCBI Gene Id: 6441
• Protein Name: Pulmonary surfactant-associated protein D
• Description of Target: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
• Uniprot ID: P35247
• Protein Accession #: NP_003010.4
• Nucleotide Accession #: NM_003019.4