Catalog No: OKEH03896
Size:96T
Price: $625.00
SKU
OKEH03896
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SFTPD ELISA Kit (Bovine) (OKEH03896) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFTPD captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Component
ComponentAmount
Anti-SFTPD Microplate96 Wells (12 x 8 Well strips)
SFTPD Lyophilized Standard2
100X Biotinylated SFTPD Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.18 ng/mL
SpecificityNatural and recombinant Bovine Pulmonary surfactant-associated protein D
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSFTPD
Gene Full Namesurfactant protein D
Alias SymbolsCOLEC7;lung surfactant protein D;PSP-D;pulmonary surfactant-associated protein D;SP-D;surfactant, pulmonary-associated protein D.
NCBI Gene Id282072
Protein NameP35246
Description of TargetPulmonary surfactant-associated protein D
Uniprot IDhttps://www.uniprot.org/uniprot/P35246
Protein Accession #NP_851369.1
Nucleotide Accession #NM_181026.2
Protein Size (# AA)369
Molecular Weight37 kDa
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