SFTPC ELISA Kit (Rat) (OKEH04271)

Catalog No: OKEH04271

Size:96T

Rat SFTPC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Sftpc ELISA Kit (Rat) (OKEH04271) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sftpc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sftpc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sftpc captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.8% (n = 20)
  Mean Inter-assay CV%: < 9.7% (n = 20)
• ELISA Kit Component:
  • Sftpc Microplate: 96 Wells (12 x 8 Well Strips)
  • Sftpc Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Sftpc Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.17 ng/mL
• Specificity: Natural and recombinant Rat Pulmonary surfactant-associated protein C
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Sftpc
• Gene Full Name: surfactant protein C
• Alias Symbols: pulmonary surfactant-associated protein C;pulmonary surfactant-associated proteolipid SPL(Val);SP-C;surfactant associated protein C;Surfactant, pulmonary-associated protein C.
• NCBI Gene Id: 50683
• Protein Name: P11685
• Description of Target: Pulmonary surfactant-associated protein C
• Uniprot ID: https://www.uniprot.org/uniprot/P11685
• Protein Accession #: NP_059038.1
• Nucleotide Accession #: NM_017342.2
• Protein Size (# AA): 194
• Molecular Weight: 21 kDa