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Catalog No: OKCD07531
Size:96WELLS
Price: $825.00
SKU
OKCD07531
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Datasheets/ManualsPrintable datasheet for SFTPC ELISA Kit (Rat) (OKCD07531)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Protein C (SP-C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Protein C (SP-C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Protein C (SP-C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Surfactant Protein C (SP-C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Protein C (SP-C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Protein C (SP-C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SFTPC Microplate96 Wells (12 x 8 Well strips)
SFTPC Lyophilized Standard2
100X SFTPC HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, lung lavage fluid and other biological fluids.
Sensitivity< 0.117ng/mL
Gene SymbolSftpc
Gene Full Namesurfactant protein C
Alias Symbolspulmonary surfactant-associated protein C;pulmonary surfactant-associated proteolipid SPL(Val);SP-C;surfactant associated protein C;Surfactant, pulmonary-associated protein C.
NCBI Gene Id50683
Protein NamePulmonary surfactant-associated protein C
Description of TargetPulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Uniprot IDP11685
Protein Accession #NP_059038.1
Nucleotide Accession #NM_017342.2
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