SFTPC ELISA Kit (Rat) (OKCA01925)

Catalog No: OKCA01925

Size:96 Wells

Rat SFTPC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology SFTPC ELISA Kit (Rat) (OKCA01925) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPC has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SFTPC captured in the well.
• ELISA Kit Range: 4.69 - 300 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	98	94-102
  EDTA Plasma (n=4)	87	83-91

• ELISA Kit Component:
  • SFTPC Microplate: 96 Wells (12 x 8 Well strips)
  • SFTPC Lyophilized Standard: 2 x 300 pg
  • 100X Biotinylated SFTPC Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.17 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: SFTPC
• Gene Full Name: Pulmonary surfactant-associated protein C
• Alias Symbols: SFTP2
• NCBI Gene Id: 100009162
• Protein Name: Pulmonary surfactant-associated protein C
• Description of Target: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
• Uniprot ID: P22398
• Protein Accession #: NP_001075791.1
• Nucleotide Accession #: NM_001082322.1