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Catalog No: OKEH08396
Size:96T
Price: $800.00
SKU
OKEH08396
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH08396
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology SFTPC ELISA Kit (Pig) (OKEH08396) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFTPC captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.9% (n = 20)
Mean Inter-assay CV%: <=8.1% (n = 20)
ELISA Kit Component
ComponentAmount
SFTPC Microplate96 Wells (12 x 8 Well strips)
SFTPC Lyophilized Standard2
100X Biotinylated SFTPC Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
::Subcellular Location: Secreted Extracellular space Surface film
::Unigene: Ssc.36281
SMR: P15785
String: 9823.ENSSSCP00000010269
::Pfam: PF08999
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.33ng/mL
SpecificityNatural and recombinant Pig Pulmonary surfactant-associated protein C
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSFTPC
Alias SymbolsPulmonary surfactant-associated proteolipid SPL(Val).
Protein NameP15785
Description of TargetPulmonary surfactant-associated protein C
Uniprot IDhttps://www.uniprot.org/uniprot/P15785
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