Catalog No: OCOA03977
Size:20UG
Price: $179.00
SKU
OCOA03977
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

SFTPB Protein Lysate (OCOA03977)

Datasheets/ManualsPrintable datasheet for SFTPB Protein Lysate (OCOA03977)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSFTPB HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSFTPB
Gene Full Namesurfactant protein B
Alias Symbols18 kDa pulmonary-surfactant protein;6 kDa protein;PSP-B;pulmonary surfactant-associated protein B;pulmonary surfactant-associated proteolipid SPL(Phe);SFTB3;SFTP3;SMDP1;SP-B.
NCBI Gene Id6439
Protein NameD6W5L6, NM_198843, NP_942140, P07988
Description of TargetHomo sapiens surfactant protein B (SFTPB), transcript variant 2, mRNA, Pulmonary surfactant-associated protein B, pulmonary surfactant-associated protein B precursor [Homo sapiens], Surfactant, pulmonary-associated protein B, isoform CRA_a
Uniprot IDhttps://www.uniprot.org/uniprot/P07988
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_942140
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_198843
Molecular Weight39.5 kDa
Write Your Own Review
You're reviewing:SFTPB Protein Lysate (OCOA03977)
Your Rating