Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCA00942
Size:96 Wells
Price: $857.00
SKU
OKCA00942
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRabbit
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat8782-93
1:29591-99
1:49489-98
1:88986-96
ELISA Kit PrincipleAviva Systems Biology SFTPB ELISA Kit (Rabbit) (OKCA00942) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for SFTPB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SFTPB captured in well.
ELISA Kit Range1.87 ng/mL-120 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9286-98
EDTA Plasma (n=4)8882-94
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level SFTPB were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level SFTPB were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
SFTPB Microplate96 Wells (12 x 8 Well strips)
SFTPB Lyophilized Standard2
100X Biotinylated SFTPB Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.46 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSFTPB
Gene Full NamePulmonary surfactant-associated protein B
Alias SymbolsSP-B, SFTP3
NCBI Gene Id100009194
Protein NamePulmonary surfactant-associated protein B
Description of TargetPulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Uniprot IDP15285
Protein Accession #NP_001075812.1
Nucleotide Accession #NM_001082343.1
Write Your Own Review
You're reviewing:SFTPB ELISA Kit (Rabbit) (OKCA00942)
Your Rating
We found other products you might like!