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Catalog No: OKCD02905
Size:96WELLS
Price: $650.00
SKU
OKCD02905
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Datasheets/ManualsPrintable datasheet for SFTPB ELISA Kit (Mouse) (OKCD02905)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Protein B (SP-B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Protein B (SP-B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Protein B (SP-B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Surfactant Protein B (SP-B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Protein B (SP-B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Protein B (SP-B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SFTPB Microplate96 Wells (12 x 8 Well strips)
SFTPB Lyophilized Standard2
100X Biotinylated SFTPB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.32 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Surfactant Associated Protein B (SPB). No significant cross-reactivity or interference between Surfactant Associated Protein B (SPB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSftpb
Gene Full Namesurfactant associated protein B
Alias SymbolsAI562151;pulmonary surfactant-associated protein B;pulmonary surfactant-associated proteolipid SPL(Phe);SF-;SF-B;Sft;Sftp;Sftp3;Sftp-3;SP;SP-B.
NCBI Gene Id20388
Protein NamePulmonary surfactant-associated protein B
Description of TargetPulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Uniprot IDP50405
Protein Accession #NP_680088.1
Nucleotide Accession #NM_147779.2
Protein Size (# AA)377
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