SFTPB ELISA Kit (Human) (OKCD07528)

Catalog No: OKCD07528

Size:96WELLS

SFTPB ELISA Kit (Human) (OKCD07528) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Protein B (SP-B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Protein B (SP-B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Protein B (SP-B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Surfactant Protein B (SP-B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Protein B (SP-B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Protein B (SP-B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SFTPB Microplate: 96 Wells (12 x 8 Well strips)
  • SFTPB Lyophilized Standard: 2
  • 100X SFTPB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.61ng/mL

Target Info

• Gene Symbol: SFTPB
• Gene Full Name: surfactant protein B
• Alias Symbols: 18 kDa pulmonary-surfactant protein;6 kDa protein;PSP-B;pulmonary surfactant-associated protein B;pulmonary surfactant-associated proteolipid SPL(Phe);SFTB3;SFTP3;SMDP1;SP-B.
• NCBI Gene Id: 6439
• Protein Name: Pulmonary surfactant-associated protein B
• Description of Target: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
• Uniprot ID: P07988
• Protein Accession #: NP_000533.3
• Nucleotide Accession #: NM_000542.3