Catalog No: OKEH01167 (Formerly GWB-EZG0DL)
Size:96 Wells
Price: $625.00
SKU
OKEH01167
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SFTPB ELISA Kit (Bovine) (OKEH01167) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFTPB captured in well.
ELISA Kit Range0.312 - 20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
SFTPB Microplate96 Wells (12 x 8 Well strips)
SFTPB Lyophilized Standard2
100X Biotinylated SFTPB Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.079 ng/mL
SpecificityNatural and recombinant Bovine Pulmonary surfactant-associated protein B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSFTPB
Alias Symbols6 kDa protein, Pulmonary surfactant-associated protein B, Pulmonary surfactant-associated proteolipid SPL(Phe), SFTP3, SP-B
NCBI Gene Id507398
Protein NamePulmonary surfactant-associated protein B
Uniprot IDP15781
Protein Accession #NP_001068779.1
Nucleotide Accession #NM_001075311.2
Protein Size (# AA)373
Molecular Weight41 kDa
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