Catalog No: OKCD06960
Size:96WELLS
Price: $600.00
SKU
OKCD06960
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for SFTPA1 ELISA Kit (Human) (OKCD06960) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pulmonary Surfactant Associated Protein A1 (SFTPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pulmonary Surfactant Associated Protein A1 (SFTPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 46.88-3,000pg/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 17.43pg/mL |
Gene Symbol | SFTPA1 |
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Gene Full Name | surfactant protein A1 |
Alias Symbols | 35 kDa pulmonary surfactant-associated protein;alveolar proteinosis protein;COLEC4;collectin-4;PSAP;PSPA;PSP-A;pulmonary surfactant-associated protein A1;SFTP1;SFTPA1B;SPA;SP-A;SPA1;SP-A1;SP-A1 beta;SP-A1 delta;SP-A1 epsilon;SP-A1 gamma;surfactant protein A1B;surfactant, pulmonary-associated protein A1A;surfactant, pulmonary-associated protein A1B. |
NCBI Gene Id | 653509 |
Protein Name | Pulmonary surfactant-associated protein A1 |
Description of Target | In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages (By similarity). |
Uniprot ID | Q8IWL2 |
Protein Accession # | NP_001087239.2 |
Nucleotide Accession # | NM_001093770.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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