SFTPA1 ELISA Kit (Horse) (OKCA01071)

Catalog No: OKCA01071

Size:96 Wells

Horse SFTPA1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Horse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	91	88-96
  1:2	96	90-102
  1:4	97	94-102
  1:8	101	96-106

• ELISA Kit Principle: Aviva Systems Biology SFTPA1 ELISA Kit (Horse) (OKCA01071) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SFTPA1 captured in the well.
• ELISA Kit Range: 6.25 - 400 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	90	87-95
  EDTA Plasma (n=4)	96	94-102

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01071 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01071 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • SFTPA1 Microplate: 96 Wells (12 x 8 Well strips)
  • SFTPA1 Lyophilized Standard: 2 x 400 ng
  • 100X Biotinylated SFTPA1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.56 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: SFTPA1
• Gene Full Name: Pulmonary surfactant-associated protein A
• Alias Symbols: SFTPA
• NCBI Gene Id: 100033826
• Protein Name: Pulmonary surfactant-associated protein A
• Description of Target: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
• Uniprot ID: Q95L88
• Protein Accession #: NP_001075281.1
• Nucleotide Accession #: NM_001081812.2