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Datasheets/Manuals | Printable datasheet for anti-SFRS2 antibody - OAAB01507 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Host | Rabbit |
Application | WB, IHC-P |
:: | Function: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. Cellular Location: Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Note=Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human SFRS2. |
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Predicted Homology Based on Immunogen Sequence | Cow; Chicken; Mouse; Pig; Rat |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Sample Type Confirmation | SFRS2 is strongly supported by BioGPS gene expression data to be expressed in K562 |
Intended Use | For research use only and not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:4000 WB: 1:1000 IHC-P: 1:50~100 |
Gene Symbol | SFRS2 |
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Alias Symbols | SC35, PR264, SC-35, SFRS2, SFRS2A, SRp30b |
NCBI Gene Id | 6427 |
Protein Name | Serine/arginine-rich splicing factor 2 |
Uniprot ID | Q01130 |
Protein Accession # | NP_001182356.1 |
Molecular Weight | 25476 Da (splicing protein is 35 KD) Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SFRS2 Antibody - N-terminal region (OAAB01507)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "SFRS2 Antibody - N-terminal region (OAAB01507)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "SFRS2 Antibody - N-terminal region (OAAB01507)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide. ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SFRS2 Antibody - N-terminal region (OAAB01507)"?
This target may also be called "SC35, PR264, SC-35, SFRS2, SFRS2A, SRp30b" in publications.
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What is the shipping cost for "SFRS2 Antibody - N-terminal region (OAAB01507)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SFRS2 Antibody - N-terminal region (OAAB01507)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SFRS2 Antibody - N-terminal region (OAAB01507)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "25476 Da (splicing protein is 35 KD) Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SFRS2 Antibody - N-terminal region (OAAB01507)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SFRS2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SFRS2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SFRS2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SFRS2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SFRS2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SFRS2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.