Catalog No: OKEH04322
Size:96T
Price: $525.00
SKU
OKEH04322
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SFRP2 ELISA Kit (Human) (OKEH04322) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFRP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFRP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFRP2 captured in the well.
ELISA Kit Range1.25-80ng/mL
ELISA Kit Recovery<= 5.9%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.9% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
SFRP2 Microplate96 Wells (12 x 8 Well Strips)
SFRP2 Standard2 x 2 ng
Sample Diluent1 x 20 mL
SFRP2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.65 ng/mL
SpecificityNatural and recombinant Human Secreted frizzled-related protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSFRP2
Gene Full Namesecreted frizzled related protein 2
Alias SymbolsFRP-2;SARP1;SARP-1;SDF-5;secreted apoptosis related protein 1;Secreted apoptosis-related protein 1;secreted frizzled-related protein 2;sFRP-2;testicular tissue protein Li 170.
NCBI Gene Id6423
Protein NameQ96HF1
Description of TargetSecreted frizzled-related protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q96HF1
Protein Accession #NP_003004.1
Nucleotide Accession #NM_003013.2
Protein Size (# AA)295
Molecular Weight33 kDa
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