SFN ELISA Kit (Bovine) (OKEH08146)

Catalog No: OKEH08146

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SFN ELISA Kit (Bovine) (OKEH08146) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFN captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.5% (n = 20)
  Mean Inter-assay CV%: <=10.7% (n = 20)
• ELISA Kit Component:
  • SFN Microplate: 96 Wells (12 x 8 Well strips)
  • SFN Lyophilized Standard: 2
  • 100X Biotinylated SFN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
  Subcellular Location: Cytoplasm Nucleus Secreted May be secreted by a non-classical secretory pathway.
  Unigene: Bt.9957
  SMR: Q0VC36
  String: 9913.ENSBTAP00000012154
  KEGG: bta:528453
  Pfam: PF00244
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine 14-3-3 protein sigma
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SFN
• Gene Full Name: stratifin
• Alias Symbols: 14-3-3 protein sigma;epithelial cell marker protein 1;Stratifin.
• NCBI Gene Id: 528453
• Protein Name: Q0VC36
• Description of Target: 14-3-3 protein sigma
• Uniprot ID: https://www.uniprot.org/uniprot/Q0VC36
• Protein Accession #: NP_001069380.1
• Nucleotide Accession #: NM_001075912.1