SETDB1 Antibody (OACA07787)

Catalog No: OACA07787

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Immunohistochemistry of paraffin-embedded human placenta at dilution 1:100

Western blot
All lanes: SETDB1 antibody at 5ug/ml
Lane 1: rat adrenal gland tissue
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 144,45 kDa
Observed band size: 143 kDa

Western blot
All lanes: SETDB1 antibody at 5ug/ml
Lane 1: rat adrenal gland tissue
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 144,45 kDa
Observed band size: 143 kDa

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Immunohistochemistry of paraffin-embedded human placenta at dilution 1:100

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Histone-lysine N-methyltransferase SETDB1 protein (1-397AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R. Nature 441:315-321(2006). [2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004).

Target Info

• Gene Symbol: SETDB1
• Gene Full Name: SET domain bifurcated histone lysine methyltransferase 1
• Alias Symbols: ERG-associated protein with a SET domain, ESET;ERG-associated protein with SET domain;ESET;H3-K9-HMTase4;histone H3-K9 methyltransferase 4;histone-lysine N-methyltransferase SETDB1;histone-lysine N-methyltransferase, H3lysine-9 specific 4;KG1T;KMT1E;lysine N-methyltransferase 1E;SET domain bifurcated 1;TDRD21;tudor domain containing 21.
• NCBI Gene Id: 9869
• Protein Name: Histone-lysine N-methyltransferase SETDB1
• Description of Target: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).
• Uniprot ID: Q15047
• Protein Accession #: NP_001138887
• Nucleotide Accession #: NM_001145415