Catalog No: OKCD09379
Size:96WELLS
Price: $750.00
SKU
OKCD09379
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Datasheets/ManualsPrintable datasheet for SETD7 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SET Domain Containing Protein 7 (SETD7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SET Domain Containing Protein 7 (SETD7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SET Domain Containing Protein 7 (SETD7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SET Domain Containing Protein 7 (SETD7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SET Domain Containing Protein 7 (SETD7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SET Domain Containing Protein 7 (SETD7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SETD7 Microplate96 Wells (12 x 8 Well strips)
SETD7 Lyophilized Standard2
100X SETD7 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.054ng/mL
Gene SymbolSETD7
Gene Full NameSET domain containing lysine methyltransferase 7
Alias SymbolsKMT7, SET7, SET9, SET7/9
NCBI Gene Id80854
Protein NameHistone-lysine N-methyltransferase SETD7
Description of TargetSETD7 is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. It plays a central role in the transcriptional activation of genes such as collagenase or insulin. It is recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. It has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the.-.-K in substrate proteins.
Uniprot IDQ8WTS6
Protein Accession #NP_001293128.1
Nucleotide Accession #NM_001306199.1
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