SERPINH1 ELISA Kit (Rat) (OKCA02556)

Catalog No: OKCA02556

Size:96 Wells

Rat SERPINH1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	87	81-93
  1:2	102	98-105
  1:4	92	88-96
  1:8	95	91-99

• ELISA Kit Principle: Aviva Systems Biology Serpinh1 ELISA Kit (Rat) (OKCA02556) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Serpinh1 captured in the well.
• ELISA Kit Range: 0.156 - 10 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	92	89-96
  EDTA Plasma (n=4)	87	83-92

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Serpinh1 Microplate: 96 Wells (12 x 8 Well strips)
  • Serpinh1 Lyophilized Standard: 2
  • 100X Biotinylated Serpinh1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 0.039 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SERPINH1
• Alias Symbols: Cbp2, Serpinh2
• NCBI Gene Id: 29345
• Protein Name: Serpin H1
• Description of Target: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
• Uniprot ID: P29457
• Protein Accession #: NP_058869
• Nucleotide Accession #: NM_017173