SERPINH1 ELISA Kit (Human) (OKCD06194)

Catalog No: OKCD06194

Size:96WELLS

SERPINH1 ELISA Kit (Human) (OKCD06194) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 47 (HSP47). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 47 (HSP47). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 47 (HSP47), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock Protein 47 (HSP47) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 125-8,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 47 (HSP47) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 47 (HSP47) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SERPINH1 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINH1 Lyophilized Standard: 2
  • 100X SERPINH1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 46pg/mL

Target Info

• Gene Symbol: SERPINH1
• Gene Full Name: serpin family H member 1
• Alias Symbols: 47 kDa heat shock protein;arsenic-transactivated protein 3;AsTP3;CBP1;CBP2;cell proliferation-inducing gene 14 protein;collagen binding protein 1;Collagen-binding protein;colligin-1;colligin-2;gp46;HSP47;OI10;PIG14;PPROM;RA-A47;rheumatoid arthritis antigen A-47;rheumatoid arthritis-related antigen RA-A47;serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1);serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2);serpin H1;serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1);SERPINH2.
• NCBI Gene Id: 871
• Protein Name: Serpin H1
• Description of Target: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
• Uniprot ID: P50454
• Protein Accession #: NP_001193943.1
• Nucleotide Accession #: NM_001207014.1