Catalog No: OCOA00022
Size:20UG
Price: $179.00
SKU
OCOA00022
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SERPING1 Protein Lysate (OCOA00022)

Datasheets/ManualsPrintable datasheet for SERPING1 Protein Lysate (OCOA00022)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSERPING1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSERPING1
Gene Full Nameserpin family G member 1
Alias SymbolsC1 esterase inhibitor;C1IN;C1INH;C1-inhibiting factor;C1-inhibitor;C1NH;complement component 1 inhibitor;epididymis secretory sperm binding protein;HAE1;HAE2;plasma protease C1 inhibitor;serine/cysteine proteinase inhibitor clade G member 1;serpin G1;serpin peptidase inhibitor clade G member 1;serpin peptidase inhibitor, clade G (C1 inhibitor), member 1.
NCBI Gene Id710
Protein NameE9KL26, NM_000062, NP_000053, P05155
Description of TargetEpididymis tissue protein Li 173, Homo sapiens serpin family G member 1 (SERPING1), transcript variant 1, mRNA, Plasma protease C1 inhibitor, plasma protease C1 inhibitor precursor [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P05155
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000053
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000062
Molecular Weight55.2 kDa
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