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Catalog No: OKCD06170
Size:96WELLS
Price: $650.00
SKU
OKCD06170
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Datasheets/ManualsPrintable datasheet for SERPING1 ELISA Kit (Rat) (OKCD06170)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1 Inhibitor (C1INH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement 1 Inhibitor (C1INH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SERPING1 Microplate96 Wells (12 x 8 Well strips)
SERPING1 Lyophilized Standard2
100X SERPING1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 2.61ng/mL
Gene SymbolSerping1
Gene Full Nameserpin family G member 1
Alias SymbolsC1 esterase inhibitor;C1 Inh;C1-IA;C1-inactivator;C1Inh;C1-inhibiting factor;plasma protease C1 inhibitor;serine (or cysteine) peptidase inhibitor, clade G, member 1;serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1, (angioedema, hereditary);serpin G1;serpin peptidase inhibitor, clade G (C1 inhibitor), member 1;serpin peptidase inhibitor, clade G, member 1.
NCBI Gene Id295703
Protein NamePlasma protease C1 inhibitor
Description of TargetActivation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein (By similarity).
Uniprot IDQ6P734
Protein Accession #NP_954524.1
Nucleotide Accession #NM_199093.1
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