Catalog No: OKCD00297 (Formerly GWB-KBBLO7)
Size:96WELLS
Price: $700.00
SKU
OKCD00297
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Datasheets/ManualsPrintable datasheet for SERPINF1 ELISA Kit (Rat) (OKCD00297)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-99%86-93%96-105%93-104%
EDTA plasma(n=5)91-103%89-101%82-93%95-105%
heparin plasma(n=5)88-101%87-96%87-94%78-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pigment Epithelium Derived Factor (PEDF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pigment Epithelium Derived Factor (PEDF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pigment Epithelium Derived Factor (PEDF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pigment Epithelium Derived Factor (PEDF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-9383
EDTA plasma(n=5)87-10197
heparin plasma(n=5)88-9992
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pigment Epithelium Derived Factor (PEDF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pigment Epithelium Derived Factor (PEDF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Serpinf1 Microplate96 Wells (12 x 8 Well Strips)
Serpinf1 Lyophilized Standard2 x 10 ng
100X Biotinylated Serpinf1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Pigment Epithelium Derived Factor (PEDF). No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSerpinf1
Gene Full Nameserpin family F member 1
Alias Symbolsalpha-2 antiplasmin;Dmrs91;Pedf;pigment epithelium-derived factor;serine (or cysteine) peptidase inhibitor, clade F, member 1;serine (or cysteine) proteinase inhibitor, clade F), member 1;serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1;sserpin family F member 1.
NCBI Gene Id287526
Protein NameAlpha-2 antiplasmin
Description of TargetAlpha-2 antiplasmin
Uniprot IDQ80ZA3
Protein Accession #NP_808788
Nucleotide Accession #NM_177927.2
Protein Size (# AA)418
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