Catalog No: OKCD00386 (Formerly GWB-KBBFC2)
Size:96WELLS
Price: $675.00
SKU
OKCD00386
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for SERPINE2 ELISA Kit (Human) (OKCD00386)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%99-105%80-105%78-91%
EDTA plasma(n=5)88-98%86-99%88-101%97-105%
heparin plasma(n=5)85-92%90-98%93-101%97-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glia Derived Nexin (GDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glia Derived Nexin (GDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glia Derived Nexin (GDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glia Derived Nexin (GDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)82-9186
EDTA plasma(n=5)86-9794
heparin plasma(n=5)85-9688
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glia Derived Nexin (GDN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glia Derived Nexin (GDN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SERPINE2 Microplate96 Wells (12 x 8 Well Strips)
SERPINE2 Lyophilized Standard2 x 10 ng
100X Biotinylated SERPINE2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glia Derived Nexin (GDN). No significant cross-reactivity or interference between Glia Derived Nexin (GDN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSERPINE2
Gene Full Nameserpin family E member 2
Alias SymbolsGDN;GDNPF;glia-derived nexin;glial-derived neurite promoting factor;peptidase inhibitor 7;PI7;PI-7;PN1;PN-1;PNI;Protease nexin 1;protease nexin I;serpin E2;serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2.
NCBI Gene Id5270
Protein NameGlia-derived nexin
Description of TargetSerine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
Uniprot IDP07093
Protein Accession #NP_001130000
Nucleotide Accession #NM_001136528.1
Protein Size (# AA)398
Write Your Own Review
You're reviewing:SERPINE2 ELISA Kit (Human) (OKCD00386)
Your Rating
We found other products you might like!