SERPINE1 ELISA Kit (Pig) (OKCA02508)

Catalog No: OKCA02508

Size:96 Wells

Pig SERPINE1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	88	83-93
  1:2	100	95-104
  1:4	90	85-94
  1:8	95	89-100

• ELISA Kit Principle: Aviva Systems Biology SERPINE1 ELISA Kit (Pig) (OKCA02508) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard SERPINE1 captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	93	87-97
  EDTA Plasma (n=4)	98	90-102

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • SERPINE1 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINE1 Lyophilized Standard: 2
  • 100X Biotinylated SERPINE1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SERPINE1
• Alias Symbols: PLANH1
• NCBI Gene Id: 396945
• Protein Name: Plasminogen activator inhibitor 1
• Description of Target: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
• Uniprot ID: P79335
• Protein Accession #: NP_999075
• Nucleotide Accession #: NM_213910