SERPIND1 ELISA Kit (Human) (OKCD06211)

Catalog No: OKCD06211

Size:96WELLS

SERPIND1 ELISA Kit (Human) (OKCD06211) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Cofactor II (HCII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparin Cofactor II (HCII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Cofactor II (HCII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heparin Cofactor II (HCII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Cofactor II (HCII) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Cofactor II (HCII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SERPIND1 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPIND1 Lyophilized Standard: 2
  • 100X SERPIND1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 0.71ng/mL

Target Info

• Gene Symbol: SERPIND1
• Gene Full Name: serpin family D member 1
• Alias Symbols: D22S673;HC2;HCF2;HCII;heparin cofactor 2;Heparin cofactor II;heparin cofactor II splice isoform;HLS2;leuserpin 2;LS2;protease inhibitor leuserpin-2;serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1;serpin D1;serpin peptidase inhibitor, clade D (heparin cofactor), member 1;THPH10.
• NCBI Gene Id: 3053
• Protein Name: Heparin cofactor 2
• Description of Target: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
• Uniprot ID: P05546
• Protein Accession #: NP_000176.2
• Nucleotide Accession #: NM_000185.3