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Catalog No: OKCD03911
Size:96WELLS
Price: $800.00
SKU
OKCD03911
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SERPINB3 Chemi-Luminescent ELISA Kit (Human) (OKCD03911)

Datasheets/ManualsPrintable datasheet for SERPINB3 Chemi-Luminescent ELISA Kit (Human) (OKCD03911)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)87-95%94-103%93-105%98-105%
EDTA Plasma (n=5)80-94%93-102%86-104%93-104%
Heparin Plasma (n=5)88-101%78-101%78-89%82-99%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Serpin B3 (SERPINB3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serpin B3 (SERPINB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serpin B3 (SERPINB3) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)93-105101
EDTA Plasma (n=5)85-9289
Heparin Plasma (n=5)88-9792
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serpin B3 (SERPINB3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serpin B3 (SERPINB3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SERPINB3 Microplate96 Wells (12 x 8 Well strips)
SERPINB3 Lyophilized Standard2
100X SERPINB3 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity0.0137 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSERPINB3
Alias SymbolsSCC, T4-A, SCCA1, SSCA1, SCCA-1, HsT1196, SCCA-PD
NCBI Gene Id6317
Protein NameSquamous cell carcinoma antigen 1
Description of TargetMay act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).
Uniprot IDP29508
Protein Accession #NP_008850
Nucleotide Accession #NM_006919
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