Catalog No: OCOA19214
Size:20UG
Price: $169.00
SKU
OCOA19214
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SERPINB2 Protein Lysate (OCOA19214)

Datasheets/ManualsPrintable datasheet for SERPINB2 Protein Lysate (OCOA19214)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSERPINB2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSERPINB2
Gene Full Nameserpin family B member 2
Alias SymbolsHsT1201;monocyte Arg-serpin;PAI;PAI2;PAI-2;placental plasminogen activator inhibitor;PLANH2;plasminogen activator inhibitor 2;plasminogen activator inhibitor, type II (arginine-serpin);plasminogen-activator inhibitor;serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2;serpin B2;serpin peptidase inhibitor, clade B (ovalbumin), member 2;urokinase inhibitor.
NCBI Gene Id5055
Protein NameNM_001143818, NP_001137290, P05120
Description of TargetHomo sapiens serpin family B member 2 (SERPINB2), transcript variant 1, mRNA, Plasminogen activator inhibitor 2, plasminogen activator inhibitor 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P05120
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001137290
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001143818
Molecular Weight46.4 kDa
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