SERPINB2 ELISA Kit (Mouse) (OKCD02781)

Catalog No: OKCD02781

Size:96WELLS

Mouse SERPINB2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  sodium citrate plasma(n=5)	86-105%	87-95%	78-103%	95-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 2 (PAI2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 2 (PAI2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen Activator Inhibitor 2 (PAI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Plasminogen Activator Inhibitor 2 (PAI2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  sodium citrate plasma(n=5)	90-104	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 2 (PAI2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 2 (PAI2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SERPINB2 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINB2 Lyophilized Standard: 2
  • 100X Biotinylated SERPINB2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: 3.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator Inhibitor 2 (PAI2). No significant cross-reactivity or interference between Plasminogen Activator Inhibitor 2 (PAI2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Serpinb2
• Gene Full Name: serine (or cysteine) peptidase inhibitor, clade B, member 2
• Alias Symbols: ova;ovalbumin;PA;PAI-2;Pl;Planh2;plasminogen activator inhibitor 2, macrophage;plasminogen activator inhibitor, type II;serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2;serine (or cysteine) proteinase inhibitor, clade B, member 2;serpin B2.
• NCBI Gene Id: 18788
• Protein Name: Plasminogen activator inhibitor 2, macrophage
• Description of Target: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. Not required for normal murine development or survival.
• Uniprot ID: P12388
• Protein Accession #: NP_001167641.1
• Nucleotide Accession #: NM_001174170.1
• Protein Size (# AA): 415