Catalog No: OKCD00194 (Formerly GWB-KBBBY7)
Size:96WELLS
Price: $700.00
SKU
OKCD00194
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for SERPINB1A ELISA Kit (Mouse) (OKCD00194) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Elastase Inhibitor (LEI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Elastase Inhibitor (LEI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Elastase Inhibitor (LEI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukocyte Elastase Inhibitor (LEI) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 15.6-1,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukocyte Elastase Inhibitor (LEI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukocyte Elastase Inhibitor (LEI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 6.4 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Leukocyte Elastase Inhibitor (LEI). No significant cross-reactivity or interference between Leukocyte Elastase Inhibitor (LEI) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | Serpinb1a |
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Gene Full Name | serine (or cysteine) peptidase inhibitor, clade B, member 1a |
Alias Symbols | 1190005M04Rik;AI325983;E;EI;EIA;ELA;ELANH2;L;LEI;leukocyte elastase inhibitor A;M/N;M/NEI;MN;MNEI;ova;ovalbumin;PI2;serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1a;serine (or cysteine) proteinase inhibitor, clade B, member 1a;serine protease inhibitor EIA;serpin B1a. |
NCBI Gene Id | 66222 |
Protein Name | Leukocyte elastase inhibitor A |
Description of Target | Regulates the activity of the neutrophil proteases and thus, forms complexes with chymotrypsin, elastase, cathepsin G and proteinase-3. |
Uniprot ID | Q9D154 |
Protein Accession # | NP_079705 |
Nucleotide Accession # | NM_025429.2 |
Protein Size (# AA) | 379 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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